Review



superscript ii rt enzyme  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    TaKaRa superscript ii rt enzyme
    Superscript Ii Rt Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/us11643695-33-29-44
    Average 86 stars, based on 1 article reviews
    superscript ii rt enzyme - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Amplification:

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ terminal sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). .. The used primer was 3′CDS primer A, and the other components and conditions were the same as those of 5′ terminal amplification. (2) Quick Amplification of cDNA Ends The first round PCR amplification was performed using the primers GSP-IL-6-4 and UPM using the cDNA synthesized previously as a template, and the 3′-RACE reaction system was added in the following order (the reaction conditions were the same as those of the 5′ ends amplification): sterilized, distilled water 31.5 μl 10 × PCR buffer 5.0 μl 25 mM MgCl2 3.0 μl 10 mM dNTP mix 1.0 μl 10 μM GSP-IL-6-4 2.0 μl 10 uM UPM 2.0 μl dC-tailed cDNA 5.0 μl Taq DNA polymerase 0.5 μl final volume 50 μl The PCR product from the first round amplification was diluted 50 times to perform the second round PCR amplification, the other systems were the same as those of the first round PCR amplification other than the primers GSP-IL-6-5 and UPM, and the reaction conditions were the same as those of 5′ amplification.

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ Terminal Sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). ..

    Sequencing:

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ terminal sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). .. The used primer was 3′CDS primer A, and the other components and conditions were the same as those of 5′ terminal amplification. (2) Quick Amplification of cDNA Ends The first round PCR amplification was performed using the primers GSP-IL-6-4 and UPM using the cDNA synthesized previously as a template, and the 3′-RACE reaction system was added in the following order (the reaction conditions were the same as those of the 5′ ends amplification): sterilized, distilled water 31.5 μl 10 × PCR buffer 5.0 μl 25 mM MgCl2 3.0 μl 10 mM dNTP mix 1.0 μl 10 μM GSP-IL-6-4 2.0 μl 10 uM UPM 2.0 μl dC-tailed cDNA 5.0 μl Taq DNA polymerase 0.5 μl final volume 50 μl The PCR product from the first round amplification was diluted 50 times to perform the second round PCR amplification, the other systems were the same as those of the first round PCR amplification other than the primers GSP-IL-6-5 and UPM, and the reaction conditions were the same as those of 5′ amplification.

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ Terminal Sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). ..

    Purification:

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ terminal sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). .. The used primer was 3′CDS primer A, and the other components and conditions were the same as those of 5′ terminal amplification. (2) Quick Amplification of cDNA Ends The first round PCR amplification was performed using the primers GSP-IL-6-4 and UPM using the cDNA synthesized previously as a template, and the 3′-RACE reaction system was added in the following order (the reaction conditions were the same as those of the 5′ ends amplification): sterilized, distilled water 31.5 μl 10 × PCR buffer 5.0 μl 25 mM MgCl2 3.0 μl 10 mM dNTP mix 1.0 μl 10 μM GSP-IL-6-4 2.0 μl 10 uM UPM 2.0 μl dC-tailed cDNA 5.0 μl Taq DNA polymerase 0.5 μl final volume 50 μl The PCR product from the first round amplification was diluted 50 times to perform the second round PCR amplification, the other systems were the same as those of the first round PCR amplification other than the primers GSP-IL-6-5 and UPM, and the reaction conditions were the same as those of 5′ amplification.

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ Terminal Sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). ..

    Reverse Transcription:

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ terminal sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). .. The used primer was 3′CDS primer A, and the other components and conditions were the same as those of 5′ terminal amplification. (2) Quick Amplification of cDNA Ends The first round PCR amplification was performed using the primers GSP-IL-6-4 and UPM using the cDNA synthesized previously as a template, and the 3′-RACE reaction system was added in the following order (the reaction conditions were the same as those of the 5′ ends amplification): sterilized, distilled water 31.5 μl 10 × PCR buffer 5.0 μl 25 mM MgCl2 3.0 μl 10 mM dNTP mix 1.0 μl 10 μM GSP-IL-6-4 2.0 μl 10 uM UPM 2.0 μl dC-tailed cDNA 5.0 μl Taq DNA polymerase 0.5 μl final volume 50 μl The PCR product from the first round amplification was diluted 50 times to perform the second round PCR amplification, the other systems were the same as those of the first round PCR amplification other than the primers GSP-IL-6-5 and UPM, and the reaction conditions were the same as those of 5′ amplification.

    Article Title:
    Article Snippet: The purified PCR product was recycled and cloned to the pMD18-T vector (TaKaRa), and positive clones were selected for sequencing. .. Amplification of IL-6 3′ Terminal Sequence of S. chuatsi (1) Synthesis and Purification of the First Strand of cDNA Reverse transcription was performed on the total extracted RNA using SUPERSCRIPT II RT enzyme and the primer 3′CDS primer A (SMARTerTM RACE cDNA Amplification Kit, Clontech). ..



    Similar Products

    90
    Thermo Fisher superscript ii rt enzyme kit
    Superscript Ii Rt Enzyme Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/superscript+ii+rt+enzyme+kit/pm40580953-407-20-25
    Average 90 stars, based on 1 article reviews
    superscript ii rt enzyme kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    TaKaRa superscript ii rt enzyme
    Superscript Ii Rt Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/us11643695-33-29-44
    Average 86 stars, based on 1 article reviews
    superscript ii rt enzyme - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript ii rt enzyme
    Superscript Ii Rt Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/pm39420148-271-23-27
    Average 90 stars, based on 1 article reviews
    superscript ii rt enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher moloney murine reverse transcriptase enzyme (superscript ii mmlv-rt
    Moloney Murine Reverse Transcriptase Enzyme (Superscript Ii Mmlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/pmc10510252-282-8-16
    Average 90 stars, based on 1 article reviews
    moloney murine reverse transcriptase enzyme (superscript ii mmlv-rt - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript tm ii reverse transcriptase (rt) enzymes
    Superscript Tm Ii Reverse Transcriptase (Rt) Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/pmc10305683-144-18-25
    Average 90 stars, based on 1 article reviews
    superscript tm ii reverse transcriptase (rt) enzymes - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript rt ii enzyme
    Superscript Rt Ii Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt+enzyme/pmc04169509-189-5-9
    Average 90 stars, based on 1 article reviews
    superscript rt ii enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results